On-line molecular imprinted solid-phase extraction flow-injection fluorescence sensor for determination of florfenicol in animal tissues

A simple and convenient on-line molecular imprinted solid-phase extraction flow-injection fluorescence sensor was developed in this study. Florfenicol (FF)-imprinted polymer was prepared by self-assembly with acrylamide (AM) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as cross-linker. The binding characteristics of the imprinted polymer to florfenicol were evaluated by equilibrium binding experiments, and the morphology of the imprinted polymer was studied by scanning electron microscope (SEM). Fluorescence intensity of 3-p-nitrylphenyl-5-(2′-sulfonophenylazo) rhodanine (4NRASP) and bovine serum albumin (BSA) was inhibited by FF. Under optimum conditions the intensity of fluorescence shows a linear relationship with the concentration of FF over the range of 1.2 × 10−6 to 2.6 × 10−5 g mL−1 with a regression equation of ΔI = 23.54 + 17.86c (c 10−6 g mL−1) (r = 0.9965, n = 6). The low detection limit of FF was found to be 3.4 × 10−7 g mL−1 according to 11 parallel determinations for the blank solution. The relative standard deviation for the determination of 2.0 × 10−6 g mL−1 FF solution was 3.5% (n = 11). This proposed sensor could be satisfactorily applied to the determination of FF in liver and meat samples.