کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1222150 | 967856 | 2010 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Enantioselective quantification of carvedilol in human plasma by HPLC in heavily medicated heart failure patients Enantioselective quantification of carvedilol in human plasma by HPLC in heavily medicated heart failure patients](/preview/png/1222150.png)
A simple, specific, sensitive, inexpensive and rapid HPLC method for enantioselective quantification of carvedilol in human plasma was developed in this study. S(−)- and R(+)-carvedilol and R(+)-propranolol as the internal standard were extracted from human plasma by liquid–liquid extraction using methyl tert-butyl ether. Enantioseparation was performed on a reverse-phase C18 Phenomenex Luna 5micron 150 mm × 2 mm column after chiral derivatization with 2,3,4,6-tetra-O-acetyl-β-d-glucopyranosyl isothiocyanate. The mobile phase was a mixture of water and acetonitrile. The peaks were detected using a fluorescence detector, where the excitation and emission wavelengths were set at 242 and 344 nm, respectively. The limits of quantification for the S(−)- and R(+)-carvedilol enantiomers were both 0.5 ng/ml. Combined intra- and inter-day variations for both enantiomers were less than 8.3%. The combined accuracy for both enantiomers ranged from 91.7 to 104.7%. This method was used to assay the carvedilol enantiomers in human plasma samples obtained from heavily medicated heart failure patients within the framework of a clinical trial.
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 52, Issue 4, 1 August 2010, Pages 636–641