کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1222197 | 1494666 | 2012 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Quantitation of slow release triptorelin in beagle dog plasma by liquid chromatography–tandem mass spectrometry Quantitation of slow release triptorelin in beagle dog plasma by liquid chromatography–tandem mass spectrometry](/preview/png/1222197.png)
A sensitive method based on liquid chromatography–tandem mass spectrometry has been developed for the determination of triptorelin levels in beagle dog plasma. Plasma samples were applied to Oasis® HLB solid-phase extraction (SPE) cartridges. Extracted samples were evaporated under a stream of nitrogen and then reconstituted with 100 μl methanol:water:formic acid (60:40:0.08, v/v/v). The separation was achieved on a Venusil MP-C18 column (2.1 mm × 50 mm, 3 μm, Agela) with a gradient elution. Detection utilized a Qtrap5500 system operated in the positive ion mode with multiple reaction monitoring of the analyte at m/z 656.5 → 249.1 and of the I.S. at m/z 510.8 → 120.1. The proposed method was validated by assessing the specificity, linearity, precision and accuracy, recovery, matrix effects, and stability. Linear calibration curves were obtained in the concentration range of 0.01–10 ng/ml (the correlation coefficients were above 0.995). The lower limit of quantification (LLOQ) of the method was 0.01 ng/ml. The method was successfully applied to a pharmacokinetic study of a slow release triptorelin formulation in beagle dogs following a single intramuscular injection.
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 66, July 2012, Pages 334–338