کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1222248 967858 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Simultaneous quantitation of levodopa and 3-O-methyldopa in human plasma by HPLC–ESI-MS/MS: Application for a pharmacokinetic study with a levodopa/benserazide formulation
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Simultaneous quantitation of levodopa and 3-O-methyldopa in human plasma by HPLC–ESI-MS/MS: Application for a pharmacokinetic study with a levodopa/benserazide formulation
چکیده انگلیسی

A sensitive and simple method was developed for the quantitation of levodopa and its metabolite 3-O-methyldopa, in human plasma, after oral administration of tablet formulations containing levodopa (200 mg) and benserazide (50 mg). The analytes were extracted by a protein precipitation procedure, using carbidopa as an internal standard. A mobile phase consisting of 0.2% formic acid and acetonitrile (94:6, v/v) was used and chromatographic separation was achieved using ACE C18 column (50 mm × 4.6 mm i.d.; 5 μm particle size). Selected reaction monitoring was performed using the fragmentation transitions m/z 198 → m/z 107, m/z 212 → m/z 166 and m/z 227 → m/z 181 for levodopa, 3-O-methyldopa and carbidopa, respectively. Calibration curves were constructed over the range 50.0–6000.0 ng/mL for levodopa and 25.0–4000.0 ng/mL for 3-O-methyldopa. The method shown to be specific, precise, accurate and provided recovery rates higher than 85% for all analytes. No matrix effect was detected in the samples. The validated method was applied in a pharmacokinetic study with a levodopa/benserazide tablet formulation in healthy volunteers.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 56, Issue 5, 15 December 2011, Pages 1094–1100
نویسندگان
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