کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1222580 | 1494670 | 2012 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Simultaneous determination of tetrandrine and fangchinoline in herbal medicine Stephania tetrandra S. Moore by liquid chromatography with electrochemical detection Simultaneous determination of tetrandrine and fangchinoline in herbal medicine Stephania tetrandra S. Moore by liquid chromatography with electrochemical detection](/preview/png/1222580.png)
A simple and selective method was developed for the simultaneous determination of tetrandrine and fangchinoline in herbal medicine by HPLC with electrochemical detection (ECD) on a bare glassy carbon electrode. The HPLC separation and ECD conditions have been optimized. The separation was carried out on a WondaSil® C18-WR column (4.6 mm × 250 mm, 5 μm), with the mobile phase of acetonitrile–ammonium acetate buffer (pH 6.5; 40 mM) (32:68, v/v) using an isocratic elution at the flow rate of 0.5 mL/min. The electrochemical detection potential was set at +0.9 V. The obtained LODs for tetrandrine and fangchinoline were 0.26 and 0.27 μmol/L, respectively. The method was successfully applied to the analysis of tetrandrine and fangchinoline contents in Stephania tetrandra S. Moore. It has been demonstrated that the LC-ECD method is an excellent technique for analysis of the herbal medicine. The mean recoveries were in the range of 95–105%, while the precision expressed as repetition of peak area was lower than 2.7%.
► Selective and sensitive method of liquid chromatography-electrochemical detection.
► Analysis of active components of tetrandrine and fangchinoline in Chinese herb.
► High sensitive with limit of detection 270 nM.
► Less interference from complex matrix in real sample.
► LC–MS identification and confirmation.
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 61, 5 March 2012, Pages 252–255