کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1222827 | 967871 | 2008 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Determination and pharmacokinetics of ascaridole in rat plasma by gas chromatography–mass spectrometry Determination and pharmacokinetics of ascaridole in rat plasma by gas chromatography–mass spectrometry](/preview/png/1222827.png)
A rapid and sensitive method for determination of ascaridole in rat plasma was developed based on gas chromatography–mass spectrometry (GC/MS). The analyte and internal standard (IS), naphthalene, were extracted from plasma with ethyl acetate and then separated by GC on a HP-5MS capillary analytical column (30 m × 0.25 mm, 0.25 μm) and determined by a quadrupole mass spectrometer detector operated under selected ion monitoring mode (SIM). Excellent linearity was found to be from 10 to 1000 ng/mL with a lower limit of quantitation (LLOQ) of 10 ng/mL. The accuracy was between 85.3% and 114.0%, and the precision was less than 14.5% (intra- and inter-day). The method was successfully applied to investigate the pharmacokinetic study of ascaridole in rats after a single oral dose of 30, 60 and 120 mg/kg, respectively.
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 48, Issue 3, 4 November 2008, Pages 997–1000