Simultaneous determination of eight active components in Chinese medicine ‘JiangYaBiFeng’ tablet by HPLC coupled with diode array detection

An effective, accurate and reliable method was developed for the simultaneous separation and determination of eight active components (baicalin, baicalein, sophoricoside, rutin, quercetin, genistein, pargyline and hydrochlorothiazide) in Chinese medicine ‘JiangYaBiFeng’ tablet (JYBF tablet) by high-performance liquid chromatography (HPLC) coupled with diode array detection (DAD). Due to the different UV characteristic of these components, different wavelengths were selected for analysis of different analytes, such as 210 nm for pargyline, 256 nm for sophoricoside, rutin, quercetin and genistein, and 280 nm for baicalin, baicalein and hydrochlorothiazide. Excellent linear behaviors over the investigated concentration ranges were observed with the values of R2 higher than 0.9990 for all analytes. The recovery rates and relative standard deviation (RSD) for all analytes at three different concentrations were 94.9–104.7% and 1.23–3.00%, respectively. The validated method was successfully applied to the simultaneously determination of these active components in ‘JiangYaBiFeng’ tablet from different production batches, indicating that the proposed method in this paper was particularly suitable for the routine analysis of JYBF tablet and its quality control.