کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1222987 1494676 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
HPTLC fingerprinting and quantification of lignans as markers in sesame oil and its polyherbal formulations
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
HPTLC fingerprinting and quantification of lignans as markers in sesame oil and its polyherbal formulations
چکیده انگلیسی

Sesamum (Sesamum indicum) seed and its oil have been in use in Indian traditional medicine, ‘Ayurveda’ since antiquity. However, there has been no attempt to standardize the polyherbal formulations containing sesamum oil as the main ingredient in terms of its active principle or marker compound. Biologically active lignans in sesamum oil are identified as the marker compound for the oil and its formulations. In this report, a simple, rapid and sensitive HPTLC method is described for the first time to identify and quantify sesamin and sesamolin, the major lignans of the sesamum oil and the method was applied to polyherbal formulations containing the oil for their quantitative estimation. The method was validated in terms of its calibration curve, limits of detection and quantification, precision, accuracy and robustness following standard protocols. The method thus developed was applied to sesamum oil and its commercial herbal formulations to quantify sesamin and sesamolin. The method for fingerprints of the formulations in the form of densitogram following charring of the chromatographic plate was also developed that could be useful for marker-based quality assurance of the polyherbal products containing sesamum oil.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 47, Issues 4–5, 5 August 2008, Pages 795–801
نویسندگان
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