کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1223463 | 967891 | 2007 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
HPLC method for the determination of nystatin in saliva for application in clinical studies
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: HPLC method for the determination of nystatin in saliva for application in clinical studies HPLC method for the determination of nystatin in saliva for application in clinical studies](/preview/png/1223463.png)
چکیده انگلیسی
An isocratic high-performance liquid chromatographic method was developed, optimized and validated for the determination of nystatin in human saliva (UV and fluorescence detection). A reversed-phase Luna⢠C18 column (25 °C), with a mobile phase of MeOH, H2O, and DMF (70:20:10, v/v/v), and a flow-rate of 0.8 ml/min were used. The elution time for nystatin was 5.8 ± 0.2 min. Calibration curves in human saliva were linear from 0.78 to 50 μg/ml. Limits of quantification were 0.78 μg/ml and 0.75 μg/ml for UV and fluorescence detection, respectively. The accuracy and precision values of intra- and inter-day variation studies were within acceptable limits, according to FDA guidelines. The described method has proved to be useful to give accurate measurements of nystatin in real samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 45, Issue 3, 5 November 2007, Pages 526-530
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 45, Issue 3, 5 November 2007, Pages 526-530
نویسندگان
Juan M. Llabot, Daniel A. Allemandi, Ruben H. Manzo, Marcela R. Longhi,