کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1223591 967895 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
HPLC method for enantioselective analysis of cloprostenol
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
HPLC method for enantioselective analysis of cloprostenol
چکیده انگلیسی
A new HPLC method for the separation and quantification of cloprostenol enantiomers was developed. The optimized separation system consisted of Chiralcel OD-RH column and acetonitrile-sodium dihydrogenphosphate (pH 3.0; 20 mM) (33:67, v/v) as the mobile phase. Baseline resolution of (±)-cloprostenol (R = 2.16) was achieved and the analysis time did not exceed 10 min. Limits of detection and quantification were units of μmol/l at 274 nm. The respective values decreased an order of magnitude at 210 nm. The R.S.D. values obtained for the retention factor, peak area and peak height of each enantiomer were less than 2%. Conditions for semipreparative separation of the enantiomers can be achieved easily just by a small adaptation of the mobile phase composition.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 46, Issue 5, 14 April 2008, Pages 892-897
نویسندگان
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