کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1223717 967899 2009 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Shell and small particles; Evaluation of new column technology
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Shell and small particles; Evaluation of new column technology
چکیده انگلیسی

The performance of 5 cm long columns packed with shell particles was compared to totally porous sub-2 μm particles in gradient and isocratic elution separations of hormones (dienogest, finasteride, gestodene, levonorgestrel, estradiol, ethinylestradiol, noretistherone acetate, bicalutamide and tibolone). Peak capacities around 140–150 could be achieved in 25 min with the 5 cm long columns. The Ascentis Express column (packed with 2.7 μm shell particles) showed similar efficiency to sub-2 μm particles under gradient conditions. Applying isocratic separation, the column of 2.7 μm shell particles had a reduced plate height minimum of approximately h = 1.6. It was much smaller than obtained with totally porous particles (h ≈ 2.8). The impedance time also proved more favorable with 2.7 μm shell particles than with totally porous particles. The influence of extra-column volume on column efficiency was investigated. The extra-column dispersion of the chromatographic system may cause a shift of the HETP curves.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 49, Issue 1, 15 January 2009, Pages 64–71
نویسندگان
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