Measurement of neurotransmitters from extracellular fluid in brain by in vivo microdialysis and chromatography–mass spectrometry

During the last three decades, a great deal of information has been discovered about chemical neurotransmission. However, the most important processes, namely the complex nature of neuronal circuitry, the “cross talk” between multiple neurotransmitter systems, and the varying effects neurochemicals have at different receptors, are still being explored. Techniques such as microdialysis are routinely employed to measure neurotransmitter levels in living tissue systems. Moreover, microdialysis studies have proven to be valuable in the investigation of neurodegenerative and psychiatric disease pathology, as well as in identifying novel drugs to treat such disorders. One particular challenge in performing these experiments is the requirement to couple microdialysis to sophisticated analytical equipment. Recently, considerable attention has been focused on the development of chromatographic–mass spectrometric techniques to provide more sensitive and accurate measurements of neurochemicals collected from in vivo microdialysis experiments. This review will provide a brief overview of the microdialysis technique, as well as how microdialysis and chromatography–mass spectrometry are being used to measure extracellular levels of neurotransmitters. The primary emphasis of this review will be on how these applications are used to measure levels of acetylcholine (ACh), dopamine, norepinephrine and γ-aminobutyric acid (GABA).