UPLC/MS for the identification of β-blockers

The β-blockers Oxprenolol, Metoprolol, Acebutolol, Atenolol, Propranolol, Pindolol, and Alprenolol were analysed by both UPLC/MS and HPLC/MS using mobile phases containing acetonitrile, TFA and either H2O or D2O. UPLC gave superior separation performance and the quality of the mass spectra were at least as good as those from HPLC.