کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1223969 | 967905 | 2006 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
LC-MS/MS determination of the HIV-1 protease inhibitor indinavir in brain and testis of mice
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: LC-MS/MS determination of the HIV-1 protease inhibitor indinavir in brain and testis of mice LC-MS/MS determination of the HIV-1 protease inhibitor indinavir in brain and testis of mice](/preview/png/1223969.png)
چکیده انگلیسی
A rapid and sensitive method for the determination of indinavir in mice brain and testis is described and validation data are provided. Indinavir and the internal standard (IS) amprenavir were isolated from homogenized tissue matrices using a mixed-mode solid-phase extraction (SPE) procedure and were then analyzed by reversed-phase liquid chromatography/tandem mass spectrometry (LC-MS/MS). The mass spectrometer in the positive-ion multiple reaction monitoring mode used pairs of ions at m/z of 614.1/421.3 for indinavir and of 506.1/245.3 for IS. The calibration curves were linear over the range 0.0012-0.0390 μmol/kg for brain and 0.39-12.50 μmol/kg for testis. Linearity, repeatability and accuracy were validated. The applicability of the method was demonstrated by assessing indinavir in brain and testis of three mice dosed with intravenous bolus administration of indinavir (16.3 μmol/kg).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 40, Issue 2, 13 February 2006, Pages 353-359
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 40, Issue 2, 13 February 2006, Pages 353-359
نویسندگان
Manuela Pereira de Oliveira, Nicolas Venisse, William Couet, Jean-Christophe Olivier,