کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1224030 967907 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The development of multiple probe microdialysis sampling in the stomach
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
The development of multiple probe microdialysis sampling in the stomach
چکیده انگلیسی
A multiple probe approach of implanting microdialysis probes into each separate tissue layer would better represent sampling from the stomach. Presently, microdialysis sampling experiments are performed with only a single probe in the submucosa to represent sampling from the stomach tissue. The focus of this research was to develop a four-probe microdialysis sampling design to simultaneously monitor the stomach lumen, mucosa, submucosa and in the blood of the rat. Due to the small outer diameter of the microdialysis probe (350 μm), implantation into each separate layer was achieved with confirmation of probe location from histological examination. To assess the significance of sampling by this approach, multiple probe microdialysis sampling was used to monitor drug absorption in the stomach. Salicylic acid, caffeine and metoprolol were individually dosed to the ligated stomach. Analysis of the dialysate samples was performed by HPLC-UV and concentration-time curves and pharmacokinetics analysis were used to determine differences between the different probe locations.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 48, Issue 1, 10 September 2008, Pages 20-26
نویسندگان
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