کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1224097 | 967910 | 2006 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Supercritical fluid chromatography–tandem mass spectrometry for fast bioanalysis of R/S-warfarin in human plasma Supercritical fluid chromatography–tandem mass spectrometry for fast bioanalysis of R/S-warfarin in human plasma](/preview/png/1224097.png)
Chiral separation for the analysis of enantiomers in biological fluids by HPLC often takes relatively long chromatography time compared to achiral analysis. The advantage of fast mass transfer in packed-column supercritical fluid chromatography (pSFC) and the high-flow compatibility of APCI-MS/MS were applied to develop a fast bioanalytical method for R/S-warfarin in human plasma. Presented here are the main challenges encountered during method development of a semi-automated liquid extraction SFC-MS/MS method. The selection of internal standard, robustness of the SFC equipment, and carryover issues are discussed. The method has high-throughput: the chromatography time is at least two-fold faster than the our fastest previous method; and the liquid/liquid extraction time of 96 samples is less than 20 min using a Tecan Genesis® RSP 100 pipetting station and a Tomtec Quadra-96® workstation. The standard curve range was 13.6–2500 ng/ml. Precision of QC concentrations from four validation runs was 7.0% for R-warfarin and 6.0% C.V. for S-warfarin; and the bias was 3.7 and 3.2% R.E., respectively. The method is sensitive, accurate, selective and robust, and was applied to a drug-interaction clinical study with rapid turnaround of sample analysis.
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 42, Issue 5, 16 November 2006, Pages 573–580