کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1224360 | 967920 | 2007 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Determination of azelnidipine in human plasma by liquid chromatography-electrospray ionization-mass spectrometry
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
A liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) method for the determination of azelnidipine in human plasma was established. Nicardipine was used as the internal standard (IS). After adjustment to a basic pH with sodium hydroxide solution (0.1Â M), plasma samples were extracted with cyclohexane-diethyl ether (1:1, v/v) and separated on a C18 column with a mobile phase of 20Â mM ammonium acetate solution-methanol-formic acid (25:75:0.5, v/v). The electrospray ionization was employed in a single quadrupole mass spectrometer for the determination. The method was linear over the concentration range of 0.05-40Â ng/ml. The lower limit of quantification (LLOQ) was 0.05Â ng/ml. The intra- and inter-run standard deviations were less than 9.5% and 11.0%, respectively. The method was successfully applied to study the pharmacokinetics of azelnidipine in healthy Chinese volunteers.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 43, Issue 2, 17 January 2007, Pages 575-579
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 43, Issue 2, 17 January 2007, Pages 575-579
نویسندگان
Li Ding, Li Li, Pengcheng Ma,