کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1224473 967923 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of salivary 17-ketosteroid sulfates using liquid chromatography–electrospray ionization-mass spectrometry
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Determination of salivary 17-ketosteroid sulfates using liquid chromatography–electrospray ionization-mass spectrometry
چکیده انگلیسی

A simple and sensitive liquid chromatography–electrospray ionization-mass spectrometric (LC–ESI-MS) method for the simultaneous quantification of representative 17-ketosteroid sulfates, dehydroepiandrosterone sulfate (DHEAS), androsterone sulfate and epiandrosterone sulfate, in human saliva has been developed and validated. The saliva was deproteinized with acetonitrile, purified using a solid-phase extraction cartridge and subjected to LC–MS. Deuterium-labeled DHEAS was used as the internal standard and quantification was based on the selected ion monitoring (SIM) mode of each deprotonated molecule. This method allowed the reproducible and accurate quantification of the salivary sulfates using a 100-μl sample; the intra- and inter-assay coefficients of variation were below 6.8 and 6.3%, respectively, and the % accuracy values were quantitative for all the sulfates. The limits of quantitation for all the sulfates were 100 pg/ml. No significant matrix effect or change in the measured value by freeze/thaw repetitions was observed. The developed method was applied to clinical studies, and produced satisfactory results.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 43, Issue 5, 11 April 2007, Pages 1782–1788
نویسندگان
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