کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1224480 967923 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Method development and validation of repaglinide in human plasma by HPLC and its application in pharmacokinetic studies
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Method development and validation of repaglinide in human plasma by HPLC and its application in pharmacokinetic studies
چکیده انگلیسی

In this study, the development and validation of a high-performance liquid chromatography (HPLC) assay for determination of repaglinide concentration in human plasma for pharmacokinetic studies is described. Plasma samples containing repaglinide and an internal standard, indomethacin were extracted with ethylacetate at pH 7.4. The recovery of repaglinide was 92% ± 55.31. Chromatographic separations were performed on Purospher® STAR C-18 analytical column (4.8 mm × 150 mm; 5 μm particle size). The mobile phase composed of acetonitrile–ammonium formate (pH 2.7; 0.01 M) (60:40, v/v). The flow rate was 1 ml/min. The retention time for repaglinide and indomethacin were approximately 6.2 and 5.3 min, respectively. Calibration curves of repaglinide were linear in the concentration range of 20–200 ng/ml in plasma. The limits of detection and quantification were 10 ng/ml and 20 ng/ml, respectively. The inter-day precision was from 5.21 to 11.84% and the intra-day precision ranged from 3.90 to 6.67%. The inter-day accuracy ranged 89.95 to 105.75% and intra-day accuracy ranged from 92.37 to 104.66%. This method was applied to determine repaglinide concentration in human plasma samples for a pharmacokinetic study.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 43, Issue 5, 11 April 2007, Pages 1831–1835
نویسندگان
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