کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1224497 | 1494677 | 2006 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
In vitro characterization of an in situ microdialysis sampling assay for elastase activity detection
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: In vitro characterization of an in situ microdialysis sampling assay for elastase activity detection In vitro characterization of an in situ microdialysis sampling assay for elastase activity detection](/preview/png/1224497.png)
چکیده انگلیسی
A microdialysis sampling method has been developed to detect the in vitro presence of a proteolytic enzyme, porcine elastase, external to a microdialysis probe. Elastase converts the substrate, succinyl(Ala)3-p-nitroanilide (suc(Ala)3-p-NA), to p-nitroaniline (p-NA). The substrate, suc(Ala)3-p-NA, was locally delivered through the microdialysis probe to external solutions containing different elastase activities (0.025-0.5 units/mL). The product, p-NA, was recovered back into the probe. Dialysates containing both suc(Ala)3-p-NA and p-NA were quantified using HPLC-UV. Different microdialysis suc(Ala)3-p-NA extraction efficiencies (EE) were observed among different elastase-containing solutions (buffer and 0.3% agar solutions). The p-NA concentrations recovered back into the microdialysis probe correlated with the elastase activity external to the microdialysis probe. The greatest fraction of p-NA recovered as compared to substrate lost occurred with the highest flow rate used (5.0 μL/min). However, the highest concentrations of p-NA recovered occurred at the lowest flow rates. This method may allow for microdialysis sampling to be used as a means to study localized enzyme activity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 40, Issue 5, 18 March 2006, Pages 1041-1047
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 40, Issue 5, 18 March 2006, Pages 1041-1047
نویسندگان
Amy J. Steuerwald, Joshua D. Villeneuve, Li Sun, Julie A. Stenken,