Determination of bovine serum albumin by a resonance light-scattering technique with the mixed-complex La(Phth)(phen)3+

A mixed-complex of La(Phth)(phen)3+ was synthesized. Resonance light-scattering characteristics of interaction between La(Phth)(phen)3+ with bovine serum albumin (BSA) were studied. When BSA was added, aggregation of La(Phth)(phen)3+ on the molecular surface of bovine serum albumin occurred in the pH 5.5-6.3, resulted in an enhanced resonance light-scattering (RLS) peak at 360 nm. The intensity of resonance light-scattering was found to be proportional to the concentration of BSA.