کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1224519 | 1494677 | 2006 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Rapid and sensitive liquid chromatography–mass spectrometry method for determination of ropinirole in human plasma Rapid and sensitive liquid chromatography–mass spectrometry method for determination of ropinirole in human plasma](/preview/png/1224519.png)
A rapid and robust liquid chromatography–mass spectrometry (LC–MS/MS) method was developed for non-ergoline dopamine D2-receptor agonist, ropinirole in human plasma using Es-citalopram oxalate as an internal standard. The method involves solid phase extraction from plasma, reversed-phase simple isocratic chromatographic conditions and mass spectrometric detection that enables a detection limit at picogram levels. The proposed method was validated with linear range of 20–1200 pg/ml. The extraction recoveries for ropinirole and internal standard were 90.45 and 65.42%, respectively. The R.S.D.% of intra-day and inter-day assay was lower than 15%. For its sensitivity and reliability, the proposed method is particularly suitable for pharmacokinetic studies.
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 40, Issue 5, 18 March 2006, Pages 1202–1208