کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1224559 | 967926 | 2006 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Trace level detection and quantitation of ethyl diazoacetate by reversed-phase high performance liquid chromatography and UV detection
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
A method using reversed-phase high performance liquid chromatography (HPLC) with UV detection has been developed and validated for the trace level (ng/mL) detection and quantitation of ethyl diazoacetate (EDA), a toxic impurity, in sample matrix. Method development included the evaluation of several analytical techniques including LC–MS and GC–MS, which in this case, proved to be unacceptable means of analysis. The chromatographic separation employed in this method utilizes a mobile phase system of acetonitrile and water with analysis carried out using UV detection at 250 nm. The final method showed excellent linearity, accuracy, repeatability, specificity and recovery when evaluated at the quantitation limit (QL) of 6 ng/mL.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 41, Issue 3, 7 June 2006, Pages 804–810
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 41, Issue 3, 7 June 2006, Pages 804–810
نویسندگان
Brian P. Axe,