کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1224867 | 967936 | 2006 | 6 صفحه PDF | دانلود رایگان |

The use of anisoin as pre-chromatographic reagent for LC analysis of guanidino compounds is proposed. The reagent reacts (5 min at 100 °C) with guanidino function and the resulting adducts can be chromatographed under reversed-phase conditions. A fluorescence detector (λex = 325 nm; λem = 435 nm) was used to detect guanidino adducts. The derivatization and chromatographic conditions were optimised by a series of experiments. Application to the determination of arginine and creatine in pharmaceuticals and arginine, guanidine, methylguanidine, guanidinosuccinic acid, β-guanidinopropionic acid, γ-guanidinobutyric acid, guanidinoacetic acid and homoarginine in human urine is described. Quantitation limits ranged from 6 to 30 fmol, except for creatine (510 fmol).
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 42, Issue 1, 11 September 2006, Pages 11–16