کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1224867 967936 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Anisoin: A useful pre-chromatographic derivatization fluorogenic reagent for LC analysis of guanidino compounds
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Anisoin: A useful pre-chromatographic derivatization fluorogenic reagent for LC analysis of guanidino compounds
چکیده انگلیسی

The use of anisoin as pre-chromatographic reagent for LC analysis of guanidino compounds is proposed. The reagent reacts (5 min at 100 °C) with guanidino function and the resulting adducts can be chromatographed under reversed-phase conditions. A fluorescence detector (λex = 325 nm; λem = 435 nm) was used to detect guanidino adducts. The derivatization and chromatographic conditions were optimised by a series of experiments. Application to the determination of arginine and creatine in pharmaceuticals and arginine, guanidine, methylguanidine, guanidinosuccinic acid, β-guanidinopropionic acid, γ-guanidinobutyric acid, guanidinoacetic acid and homoarginine in human urine is described. Quantitation limits ranged from 6 to 30 fmol, except for creatine (510 fmol).

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 42, Issue 1, 11 September 2006, Pages 11–16
نویسندگان
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