کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1224967 967940 2006 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Simultaneous determination of zonisamide, carbamazepine and carbamazepine-10,11-epoxide in infant serum by high-performance liquid chromatography
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Simultaneous determination of zonisamide, carbamazepine and carbamazepine-10,11-epoxide in infant serum by high-performance liquid chromatography
چکیده انگلیسی

This study developed a simple method for the simultaneous determination of zonisamide (ZNS), carbamazepine (CBZ) and its active metabolite, carbamazepine-10,11-epoxide (CBZE) in infant serum using reversed-phase high-performance liquid chromatograph (HPLC). The method involves a single-step protein precipitation procedure that uses no solid-phase or liquid–liquid extraction. The HPLC separation was carried out on a Cadenza CD-C18 column (3 μm, 4.6 mm × 150 mm) with potassium phosphate buffer (pH 4.6; 25 mM)–methanol–acetonitrile (65:20:15 (v/v/v)) as a mobile phase at a 1.0 ml/min flow rate: ZNS was detectable using a UV detector at 235 nm, and both CBZ and CBZE were at 215 nm. The quantification limits were established in accordance with each therapeutic range at 2.5 μg/ml for ZNS, 0.5 μg/ml for CBZ, and 0.25 μg/ml for CBZE. The respective coefficients of variation were 1.3–6.0% and 2.2–7.7% for the intra- and inter-assay.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 41, Issue 4, 16 June 2006, Pages 1386–1390
نویسندگان
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