کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1225016 967943 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development and validation of a simple reversed-phase high-performance liquid chromatography method for the determination of testosterone in serum of males
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Development and validation of a simple reversed-phase high-performance liquid chromatography method for the determination of testosterone in serum of males
چکیده انگلیسی

An isocratic high-performance liquid chromatographic method with detection at 234 nm was developed, optimized and validated for the determination of testosterone in human serum. Propylparaben was used as internal standard. A Hypersil BDS RP-C18 column (150 mm × 4.6 mm, 5 μm), was equilibrated with a mobile phase composed of acetonitrile and water (35:65, v/v) and having a flow rate of 1 ml/min. The elution time for testosterone and internal standard was approximately 11.6 and 9.9 min, respectively. Calibration curves of testosterone in serum were linear in the concentration range of 1–20 ng/ml. Limits of detection and quantification in serum were 0.4 and 1.1 ng/ml, respectively. Recovery was greater than 92%. Intra- and inter-day relative standard deviation for testosterone in serum was less than 2.1 and 3.9%, respectively. This method was applied to the determination of testosterone serum levels of 12 healthy males and data were correlated with data obtained using a radioimmunoassay method.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 41, Issue 2, 3 May 2006, Pages 527–532
نویسندگان
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