کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1225061 | 967944 | 2006 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Rapid separation and determination of resibufogenin and cinobufagin in toad venom and Liushen tablet by β-cyclodextrin modified micellar electrokinetic chromatography Rapid separation and determination of resibufogenin and cinobufagin in toad venom and Liushen tablet by β-cyclodextrin modified micellar electrokinetic chromatography](/preview/png/1225061.png)
A rapid cyclodextrin modified micellar electrokinetic chromatography (CD-MEKC) method was proposed for the determination of resibufogenin and cinobufagin in the Chinese herbal extracts from toad venom and its medicinal preparation (Liushen tablet). The two components have the close structural similarity and similar hydrophobicity, which result in poor resolution in normal MEKC. The addition of neutral β-CD to the MEKC system was found to improve the separation of the studied compounds. The effects of several CD-MEKC parameters on the resolutions were evaluated systematically. Based on the investigation, a background electrolyte solution consisting of 10 mM borate buffer adjusted to pH 8.5, 40 mM sodium dodecyl sulfate (SDS), 12 mM β-CD and 10% (v/v) of methanol was found to be optimal conditions for the fast separation. The contents of resibufogenin and cinobufagin were successfully determined within 5 min, with satisfactory repeatability and recovery.
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 41, Issue 1, 11 April 2006, Pages 124–128