Determination of phenothiazine derivatives by high performance thin-layer chromatography combined with densitometry

A high performance thin-layer chromatography (HPTLC) method combined with densitometry for determination of phenothiazine derivatives is described.Quantitation was performed in reflectance mode by using a computer-controlled densitometer Desaga CD 60. Established calibration curve (r > 0.999), precision (RDS values: 0.95–2.53%), detection limits as well as recovery values (101.1–102.8%) were found to be satisfactory.The presented method is rapid, precise and sensitive, and may be alternative to traditionally used HPLC.The method has been successfully applied in the analysis of pharmaceutical formulations.