Comparative analysis of secreted proteins from normal and preeclamptic trophoblastic cells using proteomic approaches

Preeclampsia (PE) is a pathology of pregnancy which represents the main cause of maternal and perinatal morbidity and mortality. Defective placentation is the first event of this pathology. The purpose of this study was to identify the proteins secreted by cytotrophoblastic cells (CTB) using proteomic approach that are associated with PE. Comparison of secreted proteins by mass spectrometry allowed us to identify 21 proteins which were significantly differentially secreted by control and PE CTB. One protein has been detected exclusively in supernatant of control CTB and was identified as factor XIII chain A. To determine if this observation is due to a difference of protein secretion or gene expression, its mRNA was quantified in all CTB. We found that it was significantly decreased in PE CTB compared to control. Collectively, these data suggest that decrease of factor XIII chain A might be associated with development of PE.

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► We described comparative proteomic approaches of secreted trophoblastic proteins.
► Some of them might be potent circulating markers of preeclampsia.
► For the first time, factor XIII chain A was found as a secreted trophoblastic protein.
► It is also found absent in supernatant of preeclamptic trophoblastic cells.
► It may play a role in the physiopathology of preeclampsia.