Comparative proteome analysis across non-small cell lung cancer cell lines

• Comparative proteome profiling across 23 non-small cell lung cancer cell lines.
• SILAC reference approach quantified about 4000 proteins per cell line.
• General proteome diversity larger than effects of oncogenic KRAS or EGFR mutations.
• Partial segregation of mutant cell lines in principal component analysis.
• Significantly overexpressed proteins identified in KRAS or EGFR mutant cells.

Non-small cell lung cancer (NSCLC) cell lines are widely used model systems to study molecular aspects of lung cancer. Comparative and in-depth proteome expression data across many NSCLC cell lines has not been generated yet, but would be of utility for the investigation of candidate targets and markers in oncogenesis. We employed a SILAC reference approach to perform replicate proteome quantifications across 23 distinct NSCLC cell lines. On average, close to 4000 distinct proteins were identified and quantified per cell line. These included many known targets and diagnostic markers, indicating that our proteome expression data represents a useful resource for NSCLC pre-clinical research. To assess proteome diversity within the NSCLC cell line panel, we performed hierarchical clustering and principal component analysis of proteome expression data. Our results indicate that general proteome diversity among NSCLC cell lines supersedes potential effects common to K-Ras or epidermal growth factor receptor (EGFR) oncoprotein expression. However, we observed partial segregation of EGFR or KRAS mutant cell lines for certain principal components, which reflected biological differences according to gene ontology enrichment analyses. Moreover, statistical analysis revealed several proteins that were significantly overexpressed in KRAS or EGFR mutant cell lines.

Figure optionsDownload high-quality image (147 K)Download as PowerPoint slide