کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1226133 968278 2011 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The interactome of a PTB domain-containing adapter protein, Odin, revealed by SILAC
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
The interactome of a PTB domain-containing adapter protein, Odin, revealed by SILAC
چکیده انگلیسی

Signal transduction pathways are tightly controlled by positive and negative regulators. We have previously identified Odin (also known as ankyrin repeat and sterile alpha motif domain-containing 1A; gene symbol ANKS1A) as a negative regulator of growth factor signaling; however, the mechanisms through which Odin regulates these pathways remain to be elucidated. To determine how Odin negatively regulates growth factor signaling, we undertook a proteomic approach to systematically identify proteins that interact with Odin using the SILAC strategy. In this study, we identified 18 molecules that were specifically associated in a protein complex with Odin. Our study established that the complete family of 14-3-3 proteins occur in a protein complex with Odin, which is also supported by earlier reports that identified a few members of the 14-3-3 family as Odin interactors. Among the novel protein interactors of Odin were CD2-associated protein, SH3 domain kinase binding protein 1 and DAB2 interacting protein. We confirmed 8 of the eighteen interactions identified in the Odin protein complex by co-immunoprecipitation experiments. Finally, a literature-based network analysis revealed that Odin interacting partners are involved in various cellular processes, some of which are key molecules in regulating receptor endocytosis.

Figure optionsDownload high-quality image (87 K)Download as PowerPoint slideResearch Highlights
► Odin is an adapter protein whose mechanism of action is not well understood.
► Using the SILAC strategy, we identified 18 novel proteins that associate with Odin including CD2AP, SH3KBP1, DAB2IP and members of the 14-3-3 family.
► Our interactome analysis implicates Odin in regulating endocytosis of RTKs.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Proteomics - Volume 74, Issue 3, 1 March 2011, Pages 294–303
نویسندگان
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