Probing neuropeptide signaling at the organ and cellular domains via imaging mass spectrometry

Imaging mass spectrometry (IMS) has evolved to be a promising technology due to its ability to detect a broad mass range of molecular species and create density maps for selected compounds. It is currently one of the most useful techniques to determine the spatial distribution of neuropeptides in cells and tissues. Although IMS is conceptually simple, sample preparation steps, mass analyzers, and software suites are just a few of the factors that contribute to the successful design of a neuropeptide IMS experiment. This review provides a brief overview of IMS sampling protocols, instrumentation, data analysis tools, technological advancements and applications to neuropeptide localization in neurons and endocrine tissues. Future perspectives in this field are also provided, concluding that neuropeptide IMS would greatly facilitate studies of neuronal network and biomarker discovery.This article is part of a Special Issue entitled: Imaging Mass Spectrometry: A User’s Guide to a New Technique for Biological and Biomedical Research.

This article reviews imaging mass spectrometry (IMS) principles and technology, specifically focusing on neuropeptide mapping from neural tissues and neurons.Figure optionsDownload high-quality image (95 K)Download as PowerPoint slideHighlights
► General strategies and workflow for imaging mass spectrometry (IMS) are presented.
► The current status and progress in IMS of neuropeptide (NP) analysis are reviewed.
► Sampling protocols, instrumentation, and software for IMS of NPs are discussed.
► Examples of neuropeptide imaging at the organ and cellular levels are highlighted.
► Future directions highlight key developments in IMS and the impact on NP studies.