کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1226489 968307 2011 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Usage of electrostatic eliminator reduces human keratin contamination significantly in gel-based proteomics analysis
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Usage of electrostatic eliminator reduces human keratin contamination significantly in gel-based proteomics analysis
چکیده انگلیسی

In the field of bottom-up proteomics, heavy contamination of human keratins could hinder the comprehensive protein identification, especially for the detection of low abundance proteins. In this study, we examined the keratin contamination in the four major experimental procedures in gel-based proteomic analysis including gel preparation, gel electrophoresis, gel staining, and in-gel digestion. We found that in-gel digestion procedure might be of importance corresponding to keratin contaminants compared to the other three ones. The human keratin contamination was reduced significantly by using an electrostatic eliminator during in-gel digestion, suggesting that static electricity built up on insulated experimental materials might be one of the essential causes of keratin contamination. We herein proposed a series of methods for improving experimental conditions and sample treatment in order to minimize the keratin contamination in an economical and practical way.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Proteomics - Volume 74, Issue 7, 10 June 2011, Pages 1022–1029
نویسندگان
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