کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1226593 | 1494803 | 2012 | 11 صفحه PDF | دانلود رایگان |

Chlamydia trachomatis is the most common bacterial sexually transmitted pathogen in the world. To identify new vaccine candidates a protein microarray was constructed by expressing the open reading frames (ORFs) from Chlamydia mouse pneumonitis (MoPn). C57BL/6, C3H/HeN and BALB/c mice were immunized either intranasally or intravaginally with live MoPn elementary bodies (EB). Two additional groups were immunized by the intramuscular plus subcutaneous routes with UV-treated EB, using CpG and Montanide as adjuvants to favor a Th1 response, or Alum, to elicit a Th2 response. Serum samples collected from the three strains of mice were tested in the microarray. The array included the expression of 909 proteins from the 921 ORFs of the MoPn genome and plasmid. A total of 530 ORFs were recognized by at least one serum sample. Of these, 36 reacted with sera from the three strains of mice immunized with live EB. These antigens included proteins that were previously described as immunogenic such as MOMP and HSP60. In addition, we uncovered new immunogens, including 11 hypothetical proteins. In summary, we have identified new immunodominant chlamydial proteins that can be tested for their ability to induce protection in animal models and subsequently in humans.
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► Three strains of mice, BALB/c, C3H/HeN and C57BL/6, were vaccinated with live and UV-treated Chlamydia.
► Serum samples from the immunized mice were collected over a six-month period.
► A Protein microarray representing all the open reading frames of Chlamydia was probed with the serum samples.
► We identified a group of 36 proteins that were recognized by the sera from the three strains of mice.
► These 36 immudominant antigens can now be tested for their ability to protect against chlamydial infections.
Journal: Journal of Proteomics - Volume 77, 21 December 2012, Pages 176–186