کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1228995 | 1495224 | 2015 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Multispectral studies of DNA binding, antioxidant and cytotoxic activities of a new pyranochromene derivative Multispectral studies of DNA binding, antioxidant and cytotoxic activities of a new pyranochromene derivative](/preview/png/1228995.png)
• Binding ability of a new pyranochromene derivative (3-HC) with DNA has been evaluated.
• Cytotoxic activity of 3-HC was analyzed against K562 cell line.
• The compound possesses significant cytotoxic and radical scavenging properties.
The binding properties of a new pyranochromene derivative, 2-amino-4-(3-hydroxyphenyl)-5-oxo-4H, 5H-pyrano-[3, 2-c] chromene-3-carbonitrile (3-HC) with calf thymus DNA (ctDNA) have been investigated by UV–vis absorption, circular dichroism, fluorescence spectroscopy and viscosity measurement. These results indicated that 3-HC can interact with DNA through non-intercalative mode and the intrinsic binding constant (Kb) for 3-HC with DNA was estimated to be 3.6 × 103 M−1. The antioxidant activity experiments show that 3-HC also exhibit good antioxidant activity in DPPH free radical scavenging and ferric reducing ability methods. Moreover, 3-HC exhibited cytotoxic activity against K562, human chronic myelogenous leukemia cells, with IC50 value of 146 μM and the cells responded to the treatment with mostly through apoptosis.
Figure optionsDownload as PowerPoint slide
Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy - Volume 145, 15 June 2015, Pages 353–359