Novel pyrazoline-based fluorescent probe for detecting thiols and its application in cells

• This probe can detect thiols in DMSO:HEPES = 1:1 solution at pH 7.4.
• The structure of the probe was characterized by IR, NMR and HRMS spectroscopy analysis.
• This probe can be used for living cell imaging.

A new compound, N-(4-(1,5-diphenyl-4,5-dihydro-1H-pyrazol-3-yl)phenyl)-acrylamide (probe L), was designed and synthesized as a highly sensitive and selective fluorescent probe for recognizing and detecting thiol from other amino acids. On being mixed with thiol in buffered DMSO:HEPES = 1:1 solution at pH 7.4, the probe exhibited the blue emission at 474 nm. This probe is very sensitive and displayed a linear fluorescence off–on response to thiol. The fluorescence emission of the probe is pH independent in the physiological pH range. Living cell imaging of HeLa cells confirmed its cell permeability and its ability to selectively detect thiol in cells. The structure of the probe was characterized by IR, NMR and HRMS spectroscopy analysis.

A highly sensitive and selective fluorescent probe for recognizing and detecting thiol from other amino acids was developed.Figure optionsDownload as PowerPoint slide