CuO nanosheets-enhanced flow-injection chemiluminescence system for determination of vancomycin in water, pharmaceutical and human serum

• Preparation of CuO nanosheets (NSs) by a simple one-step sonochemical method.
• Enhancement of luminol−H2O2 CL system by prepared CuO NSs.
• Determination of vancomycin using a luminol−H2O2−CuO flow-injection CL system.
• Appropriate sensitivity and selectivity of the proposed method.

A novel, rapid and sensitive CuO nanosheets (NSs) amplified flow-injection chemiluminescence (CL) system, luminol–H2O2–CuO nanosheets, was developed for determination of the vancomycin hydrochloride for the first time. It was found that vancomycin could efficiently inhibit the CL intensity of luminol–H2O2–CuO nanosheets system in alkaline medium. Under the optimum conditions, the inhibited CL intensity was linearly proportional to the concentration of vancomycin over the ranges of 0.5–18.0 and 18.0–40.0 mg L−1, with a detection limit (3σ) of 0.1 mg L−1. The precision was calculated by analyzing samples containing 5.0 mg L−1 vancomycin (n = 11) and the relative standard deviation (RSD) was 2.8%. Also, a high injection throughput of 120 sample h−1 was obtained. The CuO nanosheets were synthesized by a sonochemical method. Also, X-ray diffraction (XRD) and scanning electron microscopy (SEM) analyses were employed to characterize the CuO nanosheets. The method was successfully employed to determine vancomycin hydrochloride in environmental water samples, pharmaceutical formulation and spiked human serum.

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