کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1230538 1495240 2014 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
New insights into heat induced structural changes of pectin methylesterase on fluorescence spectroscopy and molecular modeling basis
ترجمه فارسی عنوان
بینش جدید درباره تغییرات ساختاری ناشی از گرما ناشی از متیل استراز پکتین بر طیف سنجی فلورسانس و مدلسازی مولکولی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• Spectroscopy results indicate different molecular species at high temperatures.
• Molecular modeling suggested significant conformational changes.
• The changes in PME structure over 55 °C lead to enzyme inactivation.
• Good correlation between the inactivation and fluorescence results was suggested.

Heat-induced structural changes of Aspergillus oryzae pectin methylesterase (PME) were studied by means of fluorescence spectroscopy and molecular modeling, whereas the functional enzyme stability was monitored by inactivation studies. The fluorescence spectroscopy experiments were performed at two pH value (4.5 and 7.0). At both pH values, the phase diagrams were linear, indicating the presence of two molecular species induced by thermal treatment. A red shift of 7 nm was observed at neutral pH by increasing temperature up to 60 °C, followed by a blue shift of 4 nm at 70 °C, suggesting significant conformational rearrangements. The quenching experiments using acrylamide and iodide demonstrate a more flexible conformation of enzyme with increasing temperature, especially at neutral pH. The experimental results were complemented with atomic level observations on PME model behavior after performing molecular dynamics simulations at different temperatures. The inactivation kinetics of PME in buffer solutions was fitted using a first-order kinetics model, resulting in activation energy of 241.4 ± 7.51 kJ mol−1.

3D structure of PME. The molecular motifs are represented in New Cartoon style. The Trp residues are represented in Bonds style.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy - Volume 128, 15 July 2014, Pages 15–21
نویسندگان
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