In-situ micro-FTIR spectroscopic observation on the hydration process of Poria cocos

• Micro-FTIR technique is an effect tool to location detection
• Hydrogen bonds between polysaccharide and water change with the RH increasing.
• theCH2 ⋯ H2O are formed between Poria cocos and water.
• the COH from polysaccharide bond formed COH ⋯ H2O with water molecules.

In Poria cocos, triterpene compound and polysaccharides are the main compositions. The heteropolysaccharide was identified as a linear chain of β-(1 → 3)-d-glucan, which has the strong water absorbing capacity. In order to investigate the effect of water on the structure of Poria cocos, which belongs to a kind of Polyporaceae, the Micro-Fourier transform infrared spectroscopy (micro-FTIR) technique has been employed with the ambient relative humidity (RH) increasing. The gained IR spectra are measured and analyzed in detail. Because of strong overlaps between some bands, the differential spectra and band decompositions have been applied to analyze the structural change. IR spectra show the transformation of hydrogen bonds with the RH. The blue shift of the CH2 bending vibration from 1417 to 1424 cm− 1 with the increase in RH means that the hydrogen bonds are formed between CH2 groups and water molecules at lower RH and some transferred to weak hydrogen bonds. The further study suggests that the C1OH, C2OH, and C3OH groups from polysaccharide bond formed C1OH ⋯ H2O, C2OH ⋯ H2O, and C3OH ⋯ H2O steady modes, respectively, with water molecules.

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