Application of Micro-cloud point extraction for spectrophotometric determination of Malachite green, Crystal violet and Rhodamine B in aqueous samples

• A new Micro-cloud point extraction (MCPE) method was proposed for the determination of organic dyes.
• Spectrophotometric detection and eliminating steam bath, let total analysis to be completed in a few minutes.
• Consumption of organic solvent in MCPE is in μL range and only a few mL of sample is required.
• Limit of detection of MCPE is few orders below the maximum contamination level of the analytes in water samples.

A novel, green, simple and fast method was developed for spectrophotometric determination of Malachite green, Crystal violet, and Rhodamine B in water samples based on Micro-cloud Point extraction (MCPE) at room temperature. This is the first report on the application of MCPE on dyes. In this method, to reach the cloud point at room temperature, the MCPE procedure was carried out in brine using Triton X-114 as a non-ionic surfactant. The factors influencing the extraction efficiency were investigated and optimized. Under the optimized condition, calibration curves were found to be linear in the concentration range of 0.06–0.60 mg/L, 0.10–0.80 mg/L, and 0.03–0.30 mg/L with the enrichment factors of 29.26, 85.47 and 28.36, respectively for Malachite green, Crystal violet, and Rhodamine B. Limit of detections were between 2.2 and 5.1 μg/L.

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