کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1231247 1495264 2013 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Exploring the binding mechanism of phosphoramidate derivative with DNA: Spectroscopy, calorimetry and modeling
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Exploring the binding mechanism of phosphoramidate derivative with DNA: Spectroscopy, calorimetry and modeling
چکیده انگلیسی

In this study, one of the amino phosphine ester derivatives α-(3-hydroxy-4-methoxyphenyl)-N-phenyl-α-aminophosphonate (HMPAP) was synthesized, and the molecular interaction of HMPAP with ct-DNA has been investigated by UV–Vis absorption spectra, fluorescence spectra, isothermal titration calorimetry (ITC) and molecular modeling. The binding constant (Kb) of HMPAP to ct-DNA at different temperatures were calculated from fluorescence spectra. According to the UV–Vis absorption spectra, ethidium bromide displacement studies and ITC experimental results, we can conclude that HMPAP is an intercalator. The molecular modeling results indicated that HMPAP can slide into the G–C rich region of ct-DNA. ITC data showed that ct-DNA/HMPAP binding is enthalpy controlled. Furthermore, the results obtained from molecular modeling corroborated the experimental results obtanied from spectroscopic and ITC investigations.

Figure optionsDownload as PowerPoint slideHighlights
► Medically important phosphoramidate derivative HMPAP is synthesized.
► HMPAP is intercalative binding into ctDNA helix.
► Hydrogen bonding may play an essential role in the binding of HMPAP with ctDNA.
► This binding interaction is predominantly enthalpy driven.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy - Volume 104, March 2013, Pages 492–496
نویسندگان
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