Fast and sensitive chemiluminescence assay of aminophylline in human serum using luminol–diperiodatoargentate(III) system catalyzed by coated iron nanoparticles

The CL intensity of luminol–diperiodatoargentate(III) (DPA) system is strongly enhanced by addition of iron nanoparticles (FeNPs) covered with C12E4. On injection of aminophylline into luminol–DPA–FeNPs system, the CL intensity is significantly increased. On this basis, a sensitive CL assay was developed for determination of AmP in human serum. FeNPs could catalyze the oxidation rate of luminol in the present of oxygen. Also, the CL intensity of luminol–DPA–FeNPs system is significantly increased in the presence of aminophylline (AmP). Based on this ruling, a sensitive CL assay was developed for determination of AmP in human serum. The influences of analytical variables on the CL signal were studied and optimized. Under the optimum conditions in the present of FeNPs, the CL intensity is linearly increased with AmP concentration in the range of 1.0 × 10−8–2.0 × 10−6 mol L−1. The detection limit was 9.8 × 10−9 mol L−1 AmP and the relative standard deviation for ten parallel measurements of 8.0 × 10−7 mol L−1 AmP was also 4.8%. The proposed system was successfully applied to determine AmP in human serum samples.

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► CL intensity of luminol–diperiodatoargentate(III) enhanced with Fe nanoparticles.
► FeNPs not only increase the CL intensities but also decrease the response time as well.
► The enhanced CL signal use to quantification of aminophylline in human serum.