کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1232566 | 968793 | 2010 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Synthesis of a new β-naphthothiazole monomethine cyanine dye for the detection of DNA in aqueous solution
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Synthesis of a new β-naphthothiazole monomethine cyanine dye for the detection of DNA in aqueous solution Synthesis of a new β-naphthothiazole monomethine cyanine dye for the detection of DNA in aqueous solution](/preview/png/1232566.png)
چکیده انگلیسی
Novel monomethine cyanine dye (MC) derived from β-naphthothiazole and benzothiazole has been prepared and characterized by 1H and 13C NMR, FTIR, ESIMS, elemental analyses, absorption and fluorescence spectroscopy. The dye was conveniently synthesized by the condensation of two sulfate heterocyclic quaternary salts. The interaction between calf thymus DNA (ct-DNA) in tris(hydroxymethyl)aminomethane-HCl (Tris-HCl) aqueous buffer solution and MC has been studied with spectral fluorescence method. The binding constant value has been determined by fluorescence titration of MC with ct-DNA concentrations. The result obtained is consistent with an intercalative binding interaction between MC and ct-DNA. Compared with ethidium bromide (EB), MC showed a huge fluorescence enhancement upon mixing with ct-DNA.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy - Volume 75, Issue 5, May 2010, Pages 1605-1609
Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy - Volume 75, Issue 5, May 2010, Pages 1605-1609
نویسندگان
Reda M. El-Shishtawy, Abdullah M. Asiri, Salem A. Basaif, T. Rashad Sobahi,