Preparation and characterization of the inclusion complex of Baicalin (BG) with β-CD and HP-β-CD in solution: An antioxidant ability study

The formation of the complexes of BG with β-CD and HP-β-CD was studied by UV–vis absorption spectroscopy, fluorescence spectra, Phase-solubility measurements and nuclear magnetic resonance spectroscopy (NMR) in solution. The formation constants (K) of complexes were determined by fluorescence method and Phase-solubility measurements. The results showed that the inclusion ability of β-CD and its derivatives was the order: HP-β-CD > β-CD. In addition, the experimental resulted confirmed the existence of 1:1 inclusion complex of BG with CDs.The antioxidant ability studies of BG and CDs complexes were done. The results obtained indicated that the BG/HP-β-CD complex was the most reactive form, and then was the BG/β-CD complex; the last was BG. Special configuration of complex has been proposed on NMR technique.