کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1241150 | 969171 | 2008 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular organization in protein-lipid film on the water surface studied by x-ray standing wave measurements under total external reflection
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
The x-ray standing wave method has been applied to study self-assembling processes in a protein-lipid film formed by injecting the protein-lipid mixture of alkaline phosphatase and phosphatidylinositol under the phospholipid monolayer preliminarily deposited on the water subphase by Langmuir method. X-ray standing wave measurements allowed to determine the composition of the protein-lipid film and to locate ions position in the direction normal to the film surface. The presence of trace Ni contamination incorporated in the protein-lipid film from the water subphase has been established. Numerical analysis of the X-ray standing wave fluorescence data revealed that after injection under the phospholipid monolayer, the protein-lipid mixture separated in a self-assembled manner to layered structure, molecules of alkaline phosphatase arranged themselves into a pure protein layer containing no phospholipid molecules.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Spectrochimica Acta Part B: Atomic Spectroscopy - Volume 63, Issue 12, December 2008, Pages 1399-1403
Journal: Spectrochimica Acta Part B: Atomic Spectroscopy - Volume 63, Issue 12, December 2008, Pages 1399-1403
نویسندگان
S. Zheludeva, N. Novikova, N. Stepina, E. Yurieva, O. Konovalov,