Design and characterization of novel all-solid-state potentiometric sensor array dedicated to physiological measurements

• Novel construction of all-solid-state potentiometric sensor array designed for physiological measurements has been described.
• Presented sensor array approach indicates high sensitivity, low response time and high potential stability.
• Sensor array was successfully applied to simultaneous determination of potassium, sodium, chloride ions and pH in model physiological solution and in Caco-2 cell monolayer.

A novel construction of all-solid-state potentiometric sensor array designed for physiological measurements has been presented. The planar construction and elimination of liquid phase creates broad opportunities for the modifications in the sensing part of the sensor. The designed construction is based on all-solid-state ion-selective electrodes integrated with the ionic-liquid based reference electrode. Work parameters of the sensor arrays were characterized. It has been shown that presented sensor design indicates high sensitivity (55.2±1 mV/dec, 56.3±2 mV/dec, 58.4±1 mV/dec and 53.5±1 mV/pH for sodium-, potassium-, chloride- and pH-selective electrodes, respectively in 10−5–10−1.5 M range of primary ions), low response time (t95 did not exceed 10 s), high potential stability (potential drift in 28-h measurement was ca. ±2 mV) and potential repeatability ca. ±1 mV. The system was successfully applied to the simultaneous determination of K+, Cl−, Na+ and pH in the model physiological solution and for the ion flux studies in human colon epithelium Caco-2 cell line as well.

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