کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1242489 | 969634 | 2010 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Laser induced fluorescence and photochemical derivatization for trace determination of camptothecin Laser induced fluorescence and photochemical derivatization for trace determination of camptothecin](/preview/png/1242489.png)
Laser-excited fluorescence was used for the selective determination of camptothecin in samples containing anti-cancer camptothecin-analogs (irinotecan and topotecan). The selectivity of the method was based on the UV photochemical derivatization in basic solution which increased the analyte fluorescence (337/450 nm) and eliminated fluorescence from the two campthotecin-analogs. The influence of UV exposure time and sodium hydroxide concentration was studied using an experimental design. Limit of detection was 4 × 10−10 mol L−1 with linear fluorescence response up to 1 × 10−6 mol L−1. Average recoveries of camptothecin (added to the samples to simulate a contamination) were 92 ± 4 and 94 ± 6% (n = 3) respectively in irinotecan and topotecan based pharmaceuticals.
Journal: Talanta - Volume 83, Issue 1, 15 November 2010, Pages 256–261