کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1242618 1645368 2010 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Spectrophotometric determination of isoxsuprine hydrochloride using 3-methyl-2-benzothiazolinone hydrazone hydrochloride in spiked human urine and pharmaceuticals
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Spectrophotometric determination of isoxsuprine hydrochloride using 3-methyl-2-benzothiazolinone hydrazone hydrochloride in spiked human urine and pharmaceuticals
چکیده انگلیسی
Two selective and sensitive spectrophotometric methods are proposed for the determination of isoxsuprine hydrochloride (ISX) in spiked human urine and in pharmaceuticals. The methods are based on the oxidative-coupling reaction between 3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH) and ISX in the presence of Ce(SO4)2. The novelty of the proposed reaction is the formation of two different colored chromogens at two different pHs. The resulting product at pH < 1.5 is a red colored chromogen peaking at 500 nm (method A) and that formed between the pH 3.85 and 4.15, is violet colored with an absorption maximum at 580 nm (method B). In both the methods, absorbance of the chromogen is found to increase linearly with the concentration of ISX as is corroborated by the correlation coefficients of 0.9989 and 0.9970, and the systems obey Beer's law over the ranges of 1.4-21.0 and 1.0-15.0 μg ml−1, for method A and method B, respectively. The calculated molar absorptivities are 1.08 × 104 and 1.78 × 104 l mol−1 cm−1 for method A and method B, respectively with corresponding Sandell sensitivity values of 0.0311 and 0.0190 μg cm−2. The reaction stoichiometry, in both the methods, was evaluated by the limiting logarithmic method and was found to be 1:1 (ISX:MBTH). The methods were successfully applied to the determination of ISX in spiked human urine and pharmaceutical formulation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 81, Issues 4–5, 15 June 2010, Pages 1216-1223
نویسندگان
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