کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1243239 | 1495776 | 2016 | 8 صفحه PDF | دانلود رایگان |
• Tyrosinase was immobilized via enzyme catalytic polymerization of L-DOPA.
• The biosensor exhibits performance superior to most previous reported values.
• The resultant biosensor works well both in phenol and atrazine sensing.
• The proposed biosensor works well in real water samples determination.
The facile preparation of poly(l-DOPA)-tyrosinase (PDM-Tyr) composite and its application both in substrate (phenol) and inhibitor (atrazine) sensing is reported here for the first time. Effective immobilization of enzyme is realized via in-situ entrapping Tyr in poly(l-DOPA) (PDM), which is formed by Tyr catalytic polymerization of l-DOPA. The Tyr modified electrode is simply prepared by dipping the PDM-Tyr composite on an Au electrode and then covered by Nafion. The thus-prepared Tyr-immobilized electrode exhibits excellent performance superior to most Tyr-based electrochemical biosensors, the sensitivity to phenol is as high as 5122 μA mM−1 in the linear range of 10 nM~1.25 μM, the apparent Michaelis-Menten constant (KMapp) determined as low as 3.13 μM indicates strong substrate binding and high catalytic activity of the immobilized Tyr. The biosensor also works well in atrazine biosensing, with a linear detection range of 50 ppb~30 ppm and a low detection limit of 10 ppb obtained. In addition, the biosensor shows excellent stability, precision, high sensitivity and fabrication simplicity.
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Journal: Talanta - Volume 160, 1 November 2016, Pages 125–132