کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1243255 1495776 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
An “in-electrode”-type immunosensing strategy for the detection of squamous cell carcinoma antigen based on electrochemiluminescent AuNPs/g-C3N4 nanocomposites
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
An “in-electrode”-type immunosensing strategy for the detection of squamous cell carcinoma antigen based on electrochemiluminescent AuNPs/g-C3N4 nanocomposites
چکیده انگلیسی


• A proof-of-the-concept on “in-electrode” ECL immunosensor is presented.
• The target is entirely caged by AuNPs/g-C3N4 composites tagged with antibody.
• This proposed strategy equates with extending outer Helmholtz plane of the electrode.
• The novel amplified assay is used for quantification and identification of SCCA.

A novel “in-electrode”-type electrochemiluminescence (ECL) immunosensor for the sensitive detection of squamous cell carcinoma antigen (SCCA) was constructed using magnetic graphene oxide (nanoFe3O4@GO) and Au nanoparticles/graphitic-phase carbon nitride (AuNPs/g-C3N4). The capture probe was prepared by immobilizing the primary antibody of SCCA (Ab1) on the nanoFe3O4@GO, while the AuNPs/g-C3N4 nanocomposites labelled the secondary antibody of SCCA (Ab2), which acted as a signal tag. The recognition scaffold was the following: the capture probe was immobilized onto the magnetic electrode surface that caught the target SCCA and finally allowed the immobilization of the signal tag via the interaction between antigen and antibody. Importantly, a high ECL signal could be obtained due to the unique immunocomplex, which ensured all of the g-C3N4 on the outmost plane were directly fixed onto the electrode surface and became part of the electrode surface. This resulted in an enhanced efficiency of the g-C3N4 for electrochemical luminescence, thus extending the outer Helmholtz plane (OHP) of the proposed electrode and leading to high sensitivity. Taking advantage of both nanoFe3O4@GO and AuNPs/g-C3N4, the ECL intensity was found to increase logarithmically with SCCA concentration in a wide linear range from 0.001 to 10 ng/mL and with a detection limit of 0.4 pg/mL. The proposed “in-electrode”-type ECL immunosensor was used to analyse SCCA in human serum, and satisfactory recoveries were obtained, indicating that the proposed method was promising for practical applications in the clinical diagnosis of SCCA.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 160, 1 November 2016, Pages 247–255
نویسندگان
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